10 Best Depth Of Field Microscope -Reviews & Buying Guide

Depth of field (DOF) is an important concept in microscopy. The DOF refers to a range of distance in the image that is in focus. There are many different types of microscopes, but for the purpose of this article, we will be focusing on optical microscopes. As all objects have different models and different objectives, there are several different terms used for depth of field that is interchangeably used. This article will explain these terms, as well as give information on how they can be used to improve your microscope observation experience.
It is important for people who use microscopes to understand what depth of field how affects the images produced by microscopes. For a detailed understanding of these concepts, it is helpful to understand how microscopes work.

List of 10 Best depth of field microscope: Tested by Experts

1. OMAX 5MP Digital 40X-2500X Advanced Oil NA1.25 Darkfield Trinocular Compound LED Microscope with 5.0MP Camera with Measurement, Stitching, Extended Depth of Field Software

Features :

  • Total magnification: 40X-100X-250X-400X-1000X-2500X; Eyepieces: widefield WF10X/18 and WF25X; Objectives: achromatic DIN 4X, 10X, 40X (S), 100X (S, Oil); Viewing head: 45 degrees inclined 360 degrees swiveling trinocular; Interpupillary distance: sliding adjustable 2-3/16inch ~ 2-15/16inch (55mm ~ 75mm)
  • Total magnification: 40X-100X-250X-400X-1000X-2500X; Eyepieces: widefield WF10X/18 and WF25X; Objectives: achromatic DIN 4X, 10X, 40X (S), 100X (S, Oil); Viewing head: 45 degrees inclined 360 degrees swiveling trinocular; Interpupillary distance: sliding adjustable 2-3/16inch ~ 2-15/16inch (55mm ~ 75mm)
  • Total magnification: 40X-100X-250X-400X-1000X-2500X; Eyepieces: widefield WF10X/18 and WF25X; Objectives: achromatic DIN 4X, 10X, 40X (S), 100X (S, Oil); Viewing head: 45 degrees inclined 360 degrees swiveling trinocular; Interpupillary distance: sliding adjustable 2-3/16inch ~ 2-15/16inch (55mm ~ 75mm)
  • Diopter: adjustable on both eyepiece tubes; Nosepiece: revolving quadruple; Stage: mechanical stain-resistant double layer, size: 5-1/2inch x 5-1/2inch (140mm x 140mm), translation range: 3inch x 2inch (75mm x 50mm); Photo tube adjustment range: 5/8inch (15mm); Focus: coaxial coarse and fine focus knobs on both sides, rack and pinion adjustment, with tension control; Focusing knob can be locked for observation and transportation
  • Brightfield condenser: NA1.25 Abbe condenser with iris diaphragm and filter holder, rack and pinion adjustment; Oil darkfield condenser: NA 1.36-1.25 condenser; Black palm rest on the base; Illumination: transmitted (lower), replaceable 3W LED light, variable intensity; Metal mechanical components; Power supply: 100V~240V 50/60Hz worldwide range (US and Canada power plug)
  • Digital camera: – true color 2592×1944 pixels (5M pixels) – 0.5X reduction lens to get larger field of view – 0.01 mm calibration slide: 1mm/100 division – Frame speed: 5fps at 2592×1944, 18fps at 1280×960, 60fps at 640×480 – Software compatible with Windows XP/Vista/7/8/10, Mac OS and Linux operating system – Capturing microscope images, recording live video, measuring lengths, angles, areas, editing images – USB cable included
  • 5-year warranty against manufacturing defects

Additional Info :

Item Dimensions
Height 16.141732267
Width 10.236220462
Length 7.87401574
Weight 14

2. Wireless Digital Microscope Handheld USB HD Inspection Camera 50x-1000x Magnification with Stand Compatible with iPhone, iPad, Samsung Galaxy, Android, Mac, Windows Computer

Features :

  • App Provided: Optional software for IOS, Android, Windows, MacOS X. This microscope can support Android 6.0+, iOS 9.0 or later, Windows vista/7/8/10/11 or later, MacOS X 11 or later.
  • Magnification & High Definition: 2 million pixels, 1080P HD picture quality for smartphone, 720P for computer, 50x more magnification can meet your daily needs. Built-in 8 Dimmable LEDs provide enough illuminance.
  • Easy to Carry: It is very easy to charge and the charge lasts for a long time. It makes for a very useful and fun tool to always have with you in the outdoors. You can enjoy the portable mini pocket microscope on your nature hikes.
  • A Funny Tool: This electronic microscope is more of a fixed focus magnifying glass, not a traditional microscope, Not suitable for professional serious biologists! This is definitely a very interesting thing for parents, adults, teachers, students, kids, children, collectors, testers, electronics’ repair folks, and inquisitive folks who are interested in exploring skin hair scalp trichomes and the microscopic world.
  • Not only a Microscope: More than a microscope, it is a camera, It can not only zoom in but also take photos and record videos. The ability to take video and still photos is amazing. It’s really useful when documenting plant phases throughout their lifecycle.
  • Wifi Connection for Phone: Download the software on the user manual from Google Play or APP Store; Turn on the microscope, connect the wifi (no password) which emits from the microscope; Run the app and it is easy to use.
  • USB Connection for Computer: 1. For win 10, 11, you can directly plug into the USB port and search CAMERA in WINDOWS to find it and click on. 2. WIN 7/8, please see the user manual. 3. For MacBook, please use Macbooks’ bundled software Photo Booth or Quick Time Player directly. 4. Note: Please disable the default laptop camera in Windows! And you have to change your privacy settings for the camera, which needed to be changed to permit access.
  • What You Get: 1x microscope, 1x USB cable, 1x stand, 1x base, 1x user manual, our 24-hour professional after-sales service.

Additional Info :

Color Black
Item Dimensions
Height 1.3
Width 1.3
Length 5.3
Weight 0.49

3. Grasses, Sedges, Rushes: An Identification Guide

Additional Info :

Item Dimensions
Height 8
Width 0.886
Length 5.75
Weight 1.4991433816
Release Date 2020-08-18T00:00:01Z

4. High Magnification Power Readers Reading Glasses 1.00-6.00 (Tortoise, 5.00)

Features :

  • High Magnification Power Reading Glasses – From +1.00 to +4.00, +4.50, +5.00, +6.00
  • Standard Sized Reading Glasses – One Size Fits Most! Width: 5.4 Inches, Height: 1.75 Inches, Lens Width: 1.9 Inches, Arms: 5.5 Inches.
  • Hyperextending Arms – Spring Hinge for comfort!
  • Classic styling allows for both Men and Women to enjoy these reading glasses and look great!
  • *Bonus* includes grinderPUNCH Microfiber Cleaning Pouch

Additional Info :

Color Tortoise
Item Dimensions
Height 1
Width 2
Length 6
Weight 0.125

5. Numsense! Data Science for the Layman: No Math Added

Additional Info :

Release Date 2017-02-03T17:06:46.000Z

6. LCD Digital Microscope,4.3 Inch 1080P 10 Megapixels,1-1000X Magnification Zoom Wireless USB Stereo Microscope Camera,10MP Camera Video Recorder with HD Screen

Features :

  • 【4.3 Inch High Definition Lcd Screen】 Featuring 1080P high resolution,10.0MP camera technologh and precise focus, LCD digital microscopes helps you see the clear details of tiny objects easily. 4.3 inch screen provide large visual field, for easily and accurately observe, solder etc, eyes are no longer fatigue. Support capture images(JPG) and record videos(AVI).
  • 【Long Working Distance & Stabler & Durable】Three time recording modes of 3, 5, 10 minutes, Adjustable stand, perfect for repair, soldering and large objects inspections etc. Rugged metal base provide stability, reduce vibration to keep your specimen in sharp focus.
  • 【Complete Configuration】 8 adjustable leds lights provide ample even lighting and excellent detail and optimal clarity. Yellowish color provides better contrast, helping you see the clear details easily,32G TF Card,3000 mA rechargeable battery,Can work long hours.
  • 【More Functions】2 kinds of resolutions, 7 kinds of picture quality, 5 kinds of white balance modes, adjustable exposure compensation, allowing you to observe more comprehensively.
  • 【Wide Applications & Service 】 Specially designed for electronic repairs and solder(phone, PC, watch etc ), QC inspection, insect and plants observations, fabric weaving, coins and tiny electrical components inspection etc. Also, all microscopes are covered with our 7*24 Hours service, please contact us if you have any questions.

Additional Info :

Color Black
Item Dimensions
Height 8.58
Width 3.35
Length 6.97
Weight 0.6062712205

7. EyeClops Digital Microscope & Camera with built in color screen -800X Zoom- Wireless – Use indoors & outdoors- Take pictures & video – Download to Windows PC & Mac – STEM

Features :

  • Magnify objects up to 800x!
  • Built in 2.4” Color Screen!
  • Turn off the Zoom and use as a regular Camera or video recorder!
  • Take pictures and videoUse indoors & outdoors!
  • Download via USB or MicroSD

Additional Info :

Item Dimensions
Height 11
Width 3.5
Length 9
Weight 0.9259415004
Release Date 2020-11-01T00:00:01Z

8. Elikliv EDM9 7” LCD Digital Microscope 1200X, 1080P Coin Microscope with 12MP Camera Sensor, Wired Remote, 10 LED Lights, Soldering Electronic Microscope for Adult, Compatible with Windows/Mac OS

Features :

  • 【7 Inch Rotatable Screen】Featuring 1080P high resolution,12MP camera technology and precise focus, Elikliv LCD digital microscope supports display amazing images and videos, which is convenient for easy and accurate observation and soldering. With rotatable (90 degrees) HD screen design, it is more convenient for user from different angle to view it.
  • 【Continuous 10X-1200X Magnification】Allow to zoom in and see the incredible details of observations at a magnification range from 10X to 1200X. The actual magnification differs due to the distance between camera and observations.
  • 【Adopted 10 LED Light Design】The 8 Adjustable LED illuminator ensure your specimens are clear and bright; with 2 extra auxiliary lights, Provide more excellent detail and optimal clarity and allow to capture pictures or video in some dark places.
  • 【Photos and Videos】Elikliv video microscope is not only a microscope, but also a camera, which can take pictures and record videos. You can save the images and videos that obtained during the observation process to the Micro card and output.
  • 【Hook Up To PC For Larger View 】The PC view supports Windows and Mac OS so you can observe on a larger scale and facilitate data sharing and analysis. No extra software download needed, just run the default APPs “Windows Camera” for Windows 10 and “Photo Booth” for iMac/MacBook
  • 【Wide Application, Perfect for Adults and kids Gift】 This digital microscope is very suitable for circuit board testing, watch/clock repair, textile industry, error coin identification, kids education testing, biological observation (not suitable for cells), researchers, jewelry, stamps, plants, QC inspection, it is the popular and meaningful gift for children, students, hobbyists.

Additional Info :

Color DM9

9. Carson MicroBrite Plus 60x-120x LED Lighted Pocket Microscope

Features :

  • Pocket Microscope with a Powerful 60x-120x Magnification Range
  • Precision Molded Aspheric Lenses Provide Superior Optics
  • Designed to be Compact, Lightweight and Portable.Remove the battery before storing the microscope for an extended period
  • Bright LED Light is Powered by 1 AA Battery (not included)

Additional Info :

Color Black/gray
Item Dimensions
Height 1.968503935
Width 0.787401574
Length 3.149606296
Weight 0.15
Release Date 2020-11-01T00:00:01Z

10. Immune: A Journey into the Mysterious System That Keeps You Alive

Additional Info :

Color Multicolor
Item Dimensions
Height 9.29
Width 1.42
Length 7.32
Weight 2.42
Release Date 2021-11-02T00:00:01Z

What to Consider Before Choosing the Best depth of field microscope


When it comes to picture resolution, or the clarity of detail in a magnified image of a specimen, the numerical aperture is often inversely proportional to the depth of field, and thus directly related. So, at a low numerical aperture, the resolution and depth of field will both be high, and so on. This is due to the fact that the depth of field range is determined by the distance between the lens’s angular resolution and the two intersecting points of the light path passing through the aperture.


The contrast between the specimen and its magnified picture is an important factor in determining resolution. Depths of field differ depending on the resolution and contrast of the image. Narrower specimen details necessitate a greater spatial frequency, which results in a smaller depth of field, whereas a lower contrast necessitates a larger depth of field.

Distance between collaborators

The depth of field is also influenced by the objective lens’ working distance. A short working distance results in a shallower depth of field, but a longer working distance, such as when focusing at the farthest point from the lens, results in a deeper depth of field, with practically everything in focus before that point.


When it comes to magnification, the depth of focus of the microscope is also affected, especially when using high magnification lenses like oil immersion lenses. The depth of field may be little here, yet the depth of focus may be considerable.

As a result, at higher magnifications, there is a greater risk of making a focusing error, making depth of field crucial for thick and irregularly shaped objects with complicated geometries or a variety of high and low surface spots.

In the meantime, focusing is less of a concern with low magnification lenses and infinitely thin specimens.

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